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Primer Premier is the most comprehensive software to design and analyze PCR primers. Primer Premier's search algorithm finds optimal PCR, multiplex and SNP genotyping primers with the most accurate melting temperature using the nearest neighbor thermodynamic algorithm. Primers are screened for secondary structures, dimers, hairpins, homologies and physical properties before reporting the best ones for your sequence, in ranked order. Equipped with a handy calculator, you can easily manipulate sequences and analyze the results of your primer design.

Primer Design for SNP Genotyping Assays With Primer Premier, you can load sequences from dbSNP and have the primers designed flanking the SNP selected. Hundreds of unpublished SNPs can also be loaded by specifying them as variation features in standard GenBank/dbSNP files.

After specifying the SNPs, primers can be designed to amplify them for detection using a probe-based chemistry. Top 4 Download periodically updates software information of Primer Premier 6.24 full version from the publisher, but some information may be slightly out-of-date.

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Primer Premier 6.0是由premier公司推出的一款专业引物设计软件，小编提供的这个版本适用于win7操作系统，小编还为大家带来了软件的安装方法供大家参考。 软件特色 1、主要界面是分为序列编辑窗口(Genetank)，引物设计窗口(Primer Design)，酶切分析窗口(Restriction Sites)和纹基分析窗口(Motif)。 2、该软件就是用来进行引物设计的。 3、可以简单地通过手动拖动鼠标以扩增出相应片段所需的引物。 4、在手动的任何时候，下面显示各种参数的改变和可能的二聚体、异 二聚体、发夹结构等。也可以给定条件，让软件自动搜索引物，并将引物分析结果显示出来。 5、而且进行这些操作非常简单。 安装说明 1. 先行安装 Primer Premier v6.0。 2. 启动 Primer Premier v6.0与 Primer Keygen，在 Primer Premier v6.0 程序界面的 'GeneTank' 处点击 'Active Product' 按钮，看到一个2位数的“Machine ID”。 3、把“Machine ID输入Primer Keygen，点击“Generate”生成激活码。 4、把激活码输入Primer Premier就ok了。.